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KMID : 0895820140240040001
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Journal of Oriental Rehabilitation Medicine
2014 Volume.24 No. 4 p.1 ~ p.13
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Study of Mori Fructus and Dried Mori Fructus Extracts on the Antioxidant Effect and the Inhibitory Effect on Adipocyte Differentiation
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Kim Hyung-Gu
Wang Jing-Hua
Lim Dong-Woo
Chae Hee-Sung
Chin Young-Won
Choi Han-Seok
Kim Ho-Jun
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Abstract
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Objectives : This study was to investigate the antioxidative capacity, antiobesity effect and anti-diabetes effects of Mori Fructus and dried Mori Fructus in Raw 264.7 cells and 3T3-L1 cells.
Methods : 3 different types of Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. And 3 different types of dried Mori Fructus extracts (water 100%, ethanol 30%, ethanol 100%) were used in this study. Total polyphenol compund, total favonoid compound, DPPH radical scavenging, ROS activity, NO, cell proliferation were measured in the experiment. Expressions of adipogenic transcription factors including C/EBP-α, C/EBP-β, PPAR-α, PPAR-γ, AMPK-α were analyzed by Real time PCR.
Results : Mori Fructus extracts measurements are higher than dried Mori Fructus extracts measurements at Total flavonoid compound and total flavonoid compound. Mori Fructusextracts measurements are lower than dried Mori Fructus extracts measurements at DPPH radical scavenging, ROS activity, NO. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts inhibit the expression of C/EBP-α, C/EBP-β genes. In RT-PCR analysis, there is a tendency that dried Mori Fructus extracts promote the expression of PPAR-α, PPAR-γ, AMPK-α genes.
Conclusions : Mori Fructus is effective on inhibiting the oxidation and dried Mori Fructus is effective on inhibiting the obesity and diabetes.
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KEYWORD
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Mori Fructus, Dried Mori Fructus, Antioxidative, Antiobesity, Adipocyte differ-entiation
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